The active site of all [NiFe]-hydrogenases (Hyd) has a bimetallic NiFe(CN)2CO cofactor that requires the combined action of several maturation proteins for its biosynthesis and insertion into the precursor form of the large subunit of the enzyme. Cofactor insertion is an intricately controlled process. and the large subunit of almost all Hyd enzymes has a C-terminal oligopeptide exten... https://chefesquipmenters.shop/product-category/grill-brushes/